Lindsay Fitzpatrick PhD

Education

• Postdoc - Georgia Institute of Technology (2013)
• PhD - University of Toronto (2012)
• BEng and Biosciences - McMaster University (2006)

My research program explores the interactions among biomaterials, innate immune cells and adsorbed proteins to better understand the underlying mechanisms that drive host responses to implanted biomedical materials and devices. Specifically, we are interested in defining the contributions tissue-derived proteins and other macromolecules within adsorbed protein layers on biomaterial surfaces, and studying activation of pattern recognition pathways in responding inflammatory cells, such as macrophages. The goal of our research is advance biomaterial science and improve material-tissue integration by better understanding the mechanisms driving host responses and foreign body reactions. In parallel, we are also developing rapid-throughput models for screening novel biomaterial candidates through non-invasive imaging to assess biocompatibility and host responses, as well as in vivo material characterization.

Fitzpatrick Lab trainees are eligible to apply to the Queen's Collaborative Biomedical Engineering (CBME) graduate program.

CHEE 340: Biomedical Engineering

This course will provide students with a fundamental understanding of cell biology, human physiology and the application of engineering principles (momentum and mass transfer, mechanics, materials) for the solution of medical problems. Topics include: Cell Biology, Anatomy and Physiology, Transport Phenomena in the Body, Biomechanics, Materials in Medicine, and Regenerative Medicine and Tissue Engineering.

CHEE 874: Tissue Engineering

This course is designed as a graduate level introductory course in tissue engineering: the interdisciplinary field that encompasses biology, chemistry, medical sciences and engineering to design and fabricate living systems to replace damaged or diseased tissues and organs. Topics to be discussed include: tissue anatomy, basic cell biology, cell scaffolds, cell sources and differentiation, design considerations, diffusion and mass transfer limitations, effects of external stimuli, bioreactors, methods used to evaluate the engineered product(s), and implantation. Case studies of specific tissue engineering applications will also be discussed. Students will be required to participate in, as well as lead, discussions on the course material and relevant journal articles.

Journal Articles

• McKiel LA, Ballantyne LL, Negri GL, Woodhouse KA and Fitzpatrick LE. (2023) MyD88-dependent Toll-like receptor 2 signaling modulates macrophage activation on lysate-adsorbed Teflon™ AF surfaces in an in vitro biomaterial host response model. Frontiers in Immunology, 14:1232586. DOI: 10.3389/fimmu.2023.1232586
• Kaushal A, Zhang Y, Ballantyne LL and Fitzpatrick LE (2022). TLR2-dependent signaling in the chronic macrophage response to adsorbed damage-associated molecular patterns on PTFE surfaces. Frontiers in Bioengineering and Biotechnology 10:959512. DOI: 10.3389/fbioe.2022.959512.
• McKiel LA; Woodhouse KA; Fitzpatrick LE (2020). A Macrophage Reporter Cell Assay to Examine Toll Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces. Journal of Visualized Experiments. Jan 7(155). DOI: 10.3791/60317
• McKiel LA; Woodhouse KA; Fitzpatrick LE (2020). The role of Toll-like receptor signaling in the macrophage response to implanted materials. MRS Communications 10(1): 55-68. DOI: 10.1557/mrc.2019.154 
• McKiel LA and Fitzpatrick LE (2018). Toll-like Receptor 2-Dependent NF-κB/AP-1 Activation by Damage-Associated Molecular Patterns Adsorbed on Polymeric Surfaces. ACS Biomaterials Science & Engineering 2018, 4 (11), pp 3792–3801. DOI: 10.1021/acsbiomaterials.8b00613
• Fitzpatrick LE, McDevitt TC (2015). Cell-derived matrices for tissue engineering and regenerative medicine applications. Biomaterials Science 3(1): 12-24. DOI: 10.1039/C4BM00246F 
• Fitzpatrick LE, Lisovsky A and Sefton MV. (2012) The expression of sonic hedgehog in diabetic wounds following treatment with poly(methacrylic acid-co-methyl methacrylate) beads. Biomaterials 33(21):5297-5307. DOI: 10.1016/j.biomaterials.2012.04.008.
• Fitzpatrick SD, Fitzpatrick LE, Thakur A, Mazumder MAJ and Sheardown H. (2012) Temperature-sensitive polymers for drug delivery. Expert Review of Medical Devices 9(4):339-351. DOI: 10.1586/erd.12.24.
• Fitzpatrick LE, Chan JWY and Sefton MV. (2011) On the mechanism of poly(methacrylic acid –co– methyl methacrylate)-induced angiogenesis: gene expression analysis of dTHP-1 cells. Biomaterials 32(34): 8957-8967. DOI: 10.1016/j.biomaterials.2011.08.021
• Corstorphine L and Sefton MV. (2011) Effectiveness factor and diffusion limitations in collagen gel modules containing HepG2 cells. Journal of Tissue Engineering and Regenerative Medicine 5(2): 119-129. DOI: 10.1002/term.296
• Fitzpatrick SD, Jafar Mazumder MA, Lasowski F, Fitzpatrick LE, Sheardown H. (2010) PNIPAAm-grafted-collagen as an injectable, in situ gelling, bioactive cell delivery scaffold. Biomacromolecules 11(9): 2261-2267. DOI: 10.1021/bm100299j.
• Chamberlain MD, Butler MJ, Ciucurel EC, Fitzpatrick LE, Khan OF, Leung BM, Lo C, Patel R, Velchinskaya A, Voice DN, Sefton MV. (2010) Fabrication of micro-tissues using modules of collagen gel containing cells. Journal of Visualized Experiments 13;(46). DOI: 10.3791/2177

Book Chapters

1. Antonyshyn J.A., Fitzpatrick LE. (2016) Stem Cell and Stem Cell-Derived Molecular Therapies to Enhance Dermal Wound Healing. In: Singh A., Gaharwar A. (eds) Microscale Technologies for Cell Engineering. Springer, Cham. DOI: 10.1007/978-3-319-20726-1_6
2. Fitzpatrick LE^#, Lisovsky A^#, Ciucurel EC and Sefton MV (2014) Scaffold vascularization. In Migliaresi C and Motta A (Eds.) Scaffolds for tissue engineering: biological design, materials and fabrication. Pan Stanford Publishing. (# co-first author).

Funding

• NFRF (Exploration)
• Queen's University
• NSERC (Discovery)
• Canada Foundation for Innovation (JELF)
• Ontario Ministry of Research and Innovation (ORF)
• CIHR (Project Grant)